Chelating and pendant dual-antenna effect modulated ratiometric electrochemiluminescence system for lung cancer marker assay.

Electrochemiluminescence of lanthanide coordination polymers (LCPs) has drawn researchers' torrential interest attributed to their congenital standout optical properties. Herein, a novel electrochemiluminescent (ECL) emitter LCPs Tb-norfloxacin (Tb-NFX) was firstly reported, which originated from that NFX transferred excited state energy to Tb3+ by chelating antenna effect. Moreover, a creative ratiometric ECL biosensor was established based on an emerging pendant antenna effect mechanism between luminol and Tb-NFX. Specifically, Tb-NFX was immobilized on the electrode surface and SiO 2 nanostars@luminol was introduced onto the immunosensor by the sandwich-typed immunoassay. In view of this, the luminol transferred energy to the Tb-NFX and potential resolved ratiometric strategy was implemented for self-calibrated assay target. This work innovatively demonstrates that the development of new ECL luminophores through chelating antenna effect and the establishment of a dual-potential ECL ratio measurement strategy powered by pendant antenna effect are feasible, further extending the application of LCPs in ECL immunoassays and provides new directions for the construction of ECL ratio pairs. • A lanthanide coordination polymers ECL emission is ignited based on the chelating antenna effect. • An innovative approach to first constructing ratiometric ECL system derived from the pendant antenna effect. • This strategy offers perspectives in future design of highly accurate ratiometric ECL sensing platforms. [ABSTRACT FROM AUTHOR]