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A Fluid Multivalent Magnetic Interface for High‐Performance Isolation and Proteomic Profiling of Tumor‐Derived Extracellular Vesicles.
- Source :
-
Angewandte Chemie . 5/15/2023, Vol. 135 Issue 21, p1-8. 8p. - Publication Year :
- 2023
-
Abstract
- Isolation and analysis of tumor‐derived extracellular vesicles (T‐EVs) are important for clinical cancer management. Here, we develop a fluid multivalent magnetic interface (FluidmagFace) in a microfluidic chip for high‐performance isolation, release, and protein profiling of T‐EVs. The FluidmagFace increases affinity by 105‐fold with fluidity‐enhanced multivalent binding to improve isolation efficiency by 13.9 % compared with a non‐fluid interface. Its anti‐adsorption property and microfluidic hydrodynamic shear minimize contamination, increasing detection sensitivity by two orders of magnitude. Moreover, its reversibility and expandability allow high‐throughput recovery of T‐EVs for mass spectrometric protein analysis. With the chip, T‐EVs were detected in all tested cancer samples with identification of differentially expressed proteins compared with healthy controls. The FluidmagFace opens a new avenue to isolation and release of targets for cancer diagnosis and biomarker discovery. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00448249
- Volume :
- 135
- Issue :
- 21
- Database :
- Academic Search Index
- Journal :
- Angewandte Chemie
- Publication Type :
- Academic Journal
- Accession number :
- 163565599
- Full Text :
- https://doi.org/10.1002/ange.202215337