Initiative ROS generation of Cu-doped ZIF-8 for excellent antibacterial performance.

[Display omitted] • A series of Cu-doped ZIF-8 particles are successfully synthesized. • Cu-doped ZIF-8 shows excellent antibacterial activity and low cytotoxicity. • Cu-doped ZIF-8 can catalyze oxygen to produce ROS under dark conditions. • More ROS leads to oxidative stress and membrane destruction of bacterial cells. • Low coordination copper Cu(Ⅰ) can promote electron transfer and oxygen activation. Nowadays, it is urgent to develop initiative antibacterial materials with high efficiency and low toxicity to complement or substitute conventional antibiotics and passive antibacterial materials to eliminate the ever-growing bacterial contamination. Here, a series of Cu-doped ZIF-8 materials are successfully prepared and adopted for disinfection against typical bacteria E. coli and S. aureus in the absence of light. The results show that 7 log (99.99999%) efficiency is achieved at a low dosage of 30 mg mL−1 of 1.35 atomic % Cu doped ZIF-8, orders of magnitude higher than those of Vancomycin, MIL-125-NH 2 , UiO-66-NH 2 , Cu-BTC analogs. And this concentration is confirmed as low toxicity by the cytotoxicity test. Mechanism investigation shows that reactive oxygen species (ROS) can be generated spontaneously under darkness and low-coordinated copper would contribute to the effective electron transfer for the ROS generation as revealed by the Density Functional Theory calculations. And these ROS would cause oxidative stress and membrane damage to bacterial cells and achieve excellent antibacterial properties. All these results show the potential of Cu-doped ZIF-8 in antibacterial applications, especially in environments without external energy input. [ABSTRACT FROM AUTHOR]