LY294002 对类脂 A 诱导的人冠状动脉内皮细胞缝隙 连接蛋白43表达的影响.

Objective To investigate the effect of LY294002 on the expression of connexin 43（Cx43） in human coronary endothelial cells（HCAEC） induced by lipoid A（KLA）.Methods The effects of 0,0.01,0.05,0.10,0.20 mg/L of KLA and 0,2.5,5.0,10.0,20.0 μmol/L of PI3K specific inhibitor LY294002 on the HCAEC activity were detected by CCK8 assay.HCAEC were randomly divided into the blank control group（Con group）,TLR4 agonist group（KLA group）,PI3K inhibition group（LY group） and KLA+ LY group.The protein expression levels of TLR4,PI3K and Cx43 in HCAEC of each group were detected by Western Blot.Results The survival rate of HCAEC had no statistical difference between 0.01,0.05 mg/L KLA and 0 mg/L KLA,and between 2.5 μmol/L LY294002 and 0 μmol/L LY294002（P＞0.05）,while the survival rate of HCAEC in 0.10,0.20 mg/L KLA was significantly lower than that of 0 mg/L KLA（P＜0.05）.The survival rates of HCAEC in 5.0,10.0,20.0 μmol/L LY294002 were significantly lower than those in 0 μmol/L,and the differences were statistically significant（P＜0.05）.The protein expression levels of TLR4,PI3K and Cx43 in HCAEC of the TLR4 group were significantly higher than those in the Con group, the P13K protein expression level of HCAEC in the LY group were significantly lower than those in the Con group, the TLR,P13K and Cx43 protein expression levels in the KLA+LY group were lower than those in the KLA group, and the differences were statistically significant（P＜0.05）;the TLR4 and Cx43 protein expression levels of HCAEC had no statistical difference between the LY group and Con group（P＞0.05）.Conclusion LY294002 has no significant effect on the expression of TLR4 and Cx43 proteins of HCAEC in the normal culture status.LY294002 can inhibit the high expression of Cx43 in HCAEC induced by KLA. [ABSTRACT FROM AUTHOR]