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A novel pyrimidine-based two-photon fluorogenic probe for rapidly visualizing nitroreductase activity in hypoxic cancer cells and in vivo.
- Source :
-
Sensors & Actuators B: Chemical . Sep2023, Vol. 390, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
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Abstract
- Nitroreductase (NTR) is an overexpressed enzyme in hypoxic tumor microenvironment that can be a specific recognition site for tumor diagnosis in clinical trials. Therefore, the development of probs for NTR activity with precision and instantaneity is significant. In this work, a NTR probe (ZJ) was constructed on the structure of pyrimidine derivatives with 4-animobenzyl carbamate groups. ZJ exhibited a responsive "turn-on" two-photon excited fluorescence by NTR recognition in cells and in vivo. The 4-nitrobenzyl carbamate groups in ZJ could be reduced by NTR to 4-animobenzyl carbamate groups in the presence of reduced nicotinamide adenine dinucleotide (NADH). Attributed to its self-immolation property, 4-animobenzyl carbamate would release to generate primary amine on pyrimidine, accompanied by fluorescence signal recovery of ZJ. Such responsive fluorescence turn-on process accomplished as fast as within 20 mins. The favorable stability and specificity of ZJ on NTR activity sensing was further illustrated according to the negligible interference by biological factors and the distinguishing on different NTR types. ZJ successfully visualized the distribution of over-expressed NTR in hypoxic cancer cells and in zebrafish using two-photon fluorescence microscopy, which demonstrated the potential application in studying the relationship between tumor hypoxic microenvironment and cancer. • The probe (ZJ) is two-photon fluorogenic probes for NTR detection. • ZJ can rapidly detect NTR activity. • ZJ can recognize different NTRs. • ZJ can visualize NTR in hypoxic cells and deep tissue of zebrafish. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 390
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 164155142
- Full Text :
- https://doi.org/10.1016/j.snb.2023.134015