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Clinical detection of total homocysteine in human serum using surface-enhanced Raman spectroscopy.

Clinical detection of total homocysteine in human serum using surface-enhanced Raman spectroscopy.

Authors :
Zheng, Xiao-Bing
Liu, Sheng-Hong
Panneerselvam, Rajapandiyan
Zhang, Yue-Jiao
Wang, An
Zhang, Fan-Li
Jin, Shangzhong
Li, Jian-Feng
Source :
Vibrational Spectroscopy. May2023, Vol. 126, pN.PAG-N.PAG. 1p.
Publication Year :
2023

Abstract

Cardiovascular diseases cause enormous morbidity and mortality worldwide. Excessive blood total homocysteine (tHcy) is an independent risk factor for cardiovascular diseases. Therefore, it is imperative to measure the content of tHcy in human blood. However, the currently available analytical methods have the disadvantages such as being time-consuming and expensive. Herein, we propose a rapid clinical quantification of tHcy in human serum using high-performance Ag Nanopolyhedra (Ag NPOLY) as surface-enhanced Raman spectroscopy (SERS) substrates. Generally, 80% of homocysteine (Hcy) in serum is oxidized, and only 20% of Hcy is free. To understand this behavior, we explored the reduction effect of tris(2-carboxyethyl) phosphine (TCEP) on serum Hcy at different pH. The results confirmed that our method can detect 2 µM of Hcy in human serum. As a proof-of-concept, the developed method was applied to the actual clinical detection of human serum tHcy, and the serum of 5 hyperhomocysteinemia (HHcy) patients was verified. Moreover, the obtained, results were consistent with high-performance liquid chromatography (HPLC) results. The linear correlation coefficient of the two methods was 0.9766. Importantly, our analysis can be completed within 15 min, while HCLP requires more than 1 h. Overall, we have successfully developed a rapid SERS-based quantitative method for human serum tHcy, which can provide a new solution for the clinical diagnosis of cardiovascular diseases in the future. [Display omitted] • Constructed a high-performance Ag NPOLY for the detection of tHcy in human serum. • The detection limit for Hcy is 1 µM. • Discussed the optimal condition of TCEP for Hcy reduction. • This method allows the clinical quantification of tHcy. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09242031
Volume :
126
Database :
Academic Search Index
Journal :
Vibrational Spectroscopy
Publication Type :
Academic Journal
Accession number :
164250277
Full Text :
https://doi.org/10.1016/j.vibspec.2023.103526