An ultra-small fluorescence zero-valent iron nanoclusters selectively kill gram-positive bacteria by promoting reactive oxygen species generation.

A list of the most dangerous bacteria that are multiple-drug resistance has been published by WHO, among which are various Gram-positive bacteria related with serious healthcare and community-associated infection. An effort is called for developing new strategies to combat the resistance, and nanomaterial-based approaches provide an ideal potential to mitigate the antimicrobial resistance as an alternative to antibiotics. Nanoscale zero-valent iron particles exhibited a good antimicrobial activity by triggering Fenton reaction, however, no zero-valent iron nanoclusters are developed as antimicrobial medical materials. In this work, a novel ultra-small zero-valent iron nanoclusters (usZVIN) was synthesized by one-step reduction in aqueous solutions, which exhibited bright red fluorescence at 616 nm. Interestingly, the usZVIN displayed an excellent selectively antibacterial activity against Gram-positive bacteria, and little effects on Gram-negative bacteria. The killing efficiency of usZVIN against S. aureus can reach 100 % with a concentration of 40 μg mL−1 after 1 h incubation, whereas there is no killing effect of usZVIN against E.coli even with a concentration of 900 μg mL−1 for 4 h. The antimicrobial mechanism of usZVIN was demonstrated to be the intracellular reactive oxygen species (ROS) production triggered by usZVIN due to its excellent peroxidase-like activity. Collectively, our findings suggested that usZVIN is a good medical-material candidate for fighting against Gram-positive bacterial infections, especially when we need leave beneficial Gram-negative bacteria intact. [Display omitted] • An ultra-small iron nanocluster with red fluorescence was prepared in aqueous phase. • The usZVIN were synthesized with endogenous hemin and histidine as raw materials. • The usZVIN exhibited a significant peroxidase-like activity. • The usZVIN displayed excellent antibacterial activity against Gram-positive bacteria. [ABSTRACT FROM AUTHOR]