双补丸干预雷公藤多苷致生殖损伤的作用及代谢组学分析.

Objective We aimed to (i) observe the effects of Shuangbu Pill (a compound prescription of prepared Rehmannia root and dodder seed) on reproductive damage induced by tripterygium glycosides in rats and ( ii) explore its regulatory mechanism by metabolomic analysis. Methods Using the random number table method, 21 male 4-week-old Sprague-Dawley rats were randomly divided into the normal group, the tripterygium glycosides group (12. 5 mg / kg), and the Shuangbu Pill group (3. 125 g / kg) (7 rats per group) and gavaged continuously for 12 weeks. The serum levels of testosterone (T), follicle stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2 ) were measured by ELISA; sperm density and sperm motility were measured by an automatic sperm quality analyzer; histopathological changes of the testis and epididymis were observed by HE staining; ultrahigh-performance liquid chromatography coupled with tandem time-of-flight mass spectrometry combined with multivariate statistical method was used to detect and identify testicular tissue metabolites; and the Metabo Analyst platform was used for metabolic pathway analysis. Results Compared with the tripterygium glycosides group, the Shuangbu Pill could increase serum T levels ( P < 0. 01), improve the sperm density and sperm motility ( P < 0. 01), increase the number of spermatogenic cells in testicular tissue, reduce spermatogenic cell cavitation, and increase the number of epididymal sperm. According to our metabonomic analysis, after treatment with Shuangbu Pill, the levels of 19 differential metabolites related to reproductive damage caused by tripterygium glycosides could be significantly restored, which are mainly involved in glutathione metabolism and glycerophospholipid metabolism. Conclusion Shuangbu Pill can significantly reduce the reproductive damage caused by tripterygium glycosides, possibly through regulating glutathione metabolism and glycerophospholipid metabolism. [ABSTRACT FROM AUTHOR]