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Brittle culm 25, which encodes an UDP-xylose synthase, affects cell wall properties in rice.

Authors :
Siliang Xu
Mengchen Zhang
Junhua Ye
Dongxiu Hu
Yuanyuan Zhang
Zhen Li
Junrong Liu
Yanfei Sun
Shan Wang
Xiaoping Yuan
Yue Feng
Qun Xu
Xinghua Wei
Dali Zeng
Yaolong Yang
Source :
Crop Journal (2095-5421). Jun2023, Vol. 11 Issue 3, p733-743. 11p.
Publication Year :
2023

Abstract

Because plant mechanical strength influences plant growth and development, the regulatory mechanisms underlying cell-wall synthesis deserve investigation. Rice mutants are useful for such research. We have identified a novel brittle culm 25 (bc25) mutant with reduced growth and partial sterility. BC25 encodes an UDP-glucuronic acid decarboxylase involved in cellulose synthesis and belongs to the UXS family. A single-nucleotide mutation in BC25 accounts for its altered cell morphology and cellwall composition. Transmission electron microscopy analysis showed that the thickness of the secondary cell wall was reduced in bc25. Monosaccharide analysis revealed significant increases in content of rhamnose and arabinose but not of other monosaccharides, indicating that BC25 was involved in xylose synthesis with some level of functional redundancy. Enzymatic assays suggested that BC25 functions with high activity to interconvert UDP-glucuronic acid (UDP-GlcA) and UDP-xylose. GUS staining showed that BC25 was ubiquitously expressed with higher expression in culm, root and sheath, in agreement with that shown by quantitative real-time (qRT)-PCR. RNA-seq further suggested that BC25 is involved in sugar metabolism. We conclude that BC25 strongly influences rice cell wall formation. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20955421
Volume :
11
Issue :
3
Database :
Academic Search Index
Journal :
Crop Journal (2095-5421)
Publication Type :
Academic Journal
Accession number :
164425661
Full Text :
https://doi.org/10.1016/j.cj.2022.11.011