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Feasibility study of direct CT lymphangiography in mice: comparison with interstitial CT/MR lymphangiography.
- Source :
-
European Radiology . Jul2023, Vol. 33 Issue 7, p5028-5036. 9p. 1 Color Photograph, 3 Black and White Photographs, 2 Charts, 1 Graph. - Publication Year :
- 2023
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Abstract
- Objectives: To establish a CT lymphangiography method in mice via direct lymph node puncture. Methods: We injected healthy mice (n = 8) with 50 µl of water-soluble iodine contrast agent (iomeprol; iodine concentration, 350 mg/mL) subcutaneously into the left-rear foot pad (interstitial injection) and 20 µl of the same contrast agent directly into the popliteal lymph node (direct puncture) 2 days later. Additionally, we performed interstitial MR lymphangiography on eight mice as a control group. We calculated the contrast ratio for each lymph node and visually assessed the depiction of lymph nodes and lymphatic vessels on a three-point scale. Results: The contrast ratios of 2-min post-injection images of sacral and lumbar–aortic lymph nodes were 20.7 ± 16.6 (average ± standard deviation) and 17.1 ± 12.0 in the direct puncture group, which were significantly higher than those detected in the CT or MR interstitial lymphangiography groups (average, 1.8–3.6; p = 0.008–0.019). The visual assessment scores for sacral lymph nodes, lumbar–aortic lymph nodes, and cisterna chyli were significantly better in the direct puncture group than in the CT interstitial injection group (p = 0.036, 0.009 and 0.001, respectively). The lymphatic vessels between these structures were significantly better scored in direct puncture group than in the CT or MR interstitial lymphangiography groups at 2 min after injection (all p ≤ 0.05). Conclusions: In CT lymphangiography in mice, the direct lymph node puncture provides a better delineation of the lymphatic pathways than the CT/MR interstitial injection method. Key Points: •The contrast ratios of 2-min post-injection images in the direct CT lymphangiography group were significantly higher than those of CT/MR interstitial lymphangiography groups. •The visibility of lymphatic vessels in subjective analysis in the direct CT lymphangiography group was significantly better in the direct puncture group than in the CT/MR interstitial lymphangiography groups. •CT lymphangiography with direct lymph node puncture can provide excellent lymphatic delineation with contrast being maximum at 2 min after injection. [ABSTRACT FROM AUTHOR]
- Subjects :
- *LYMPHANGIOGRAPHY
*LYMPH nodes
*CONTRAST media
*FEASIBILITY studies
*MICE
Subjects
Details
- Language :
- English
- ISSN :
- 09387994
- Volume :
- 33
- Issue :
- 7
- Database :
- Academic Search Index
- Journal :
- European Radiology
- Publication Type :
- Academic Journal
- Accession number :
- 164491458
- Full Text :
- https://doi.org/10.1007/s00330-023-09423-4