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HPLC-CAD as a supplementary method for the quantification of related structure impurities for the purity assessment of organic CRMs.
- Source :
-
Analytical & Bioanalytical Chemistry . Jul2023, Vol. 415 Issue 17, p3375-3384. 10p. - Publication Year :
- 2023
-
Abstract
- In organic purity assessment, chromatography separation with a suitable detector is required. Diode array detection (DAD) has been a widely used technique for high-performance liquid chromatography (HPLC) analyses, but its application is limited to compounds with sufficient UV chromophores. Charged aerosol detector (CAD), as a mass-dependent detector, is advantageous for providing a nearly uniform response for analytes, regardless of their structures. In this study, 11 non-volatile compounds with/without UV chromophores were analyzed by CAD using continuous direct injection mode. The RSDs of CAD responses were within 17%. For saccharides and bisphenols, especially, the RSDs were lower (2.12% and 8.14%, respectively). Since bisphenols exist in UV chromophores, their HPLC–DAD responses were studied and compared with CAD responses, with CAD showing a more uniform response. Besides, the key parameters of HPLC-CAD were optimized and the developed method was verified using a Certified Reference Material (CRM, dulcitol, GBW06144). The area normalization result of dulcitol measured by HPLC-CAD was 99.89% ± 0.02% (n = 6), consistent with the certified value of 99.8% ± 0.2% (k = 2). The result of this work indicated that the HPLC-CAD method could be a good complementary tool to traditional techniques for the purity assessment of organic compounds, especially for compounds lacking UV chromophores. [ABSTRACT FROM AUTHOR]
- Subjects :
- *HIGH performance liquid chromatography
*CHROMOPHORES
*BISPHENOLS
Subjects
Details
- Language :
- English
- ISSN :
- 16182642
- Volume :
- 415
- Issue :
- 17
- Database :
- Academic Search Index
- Journal :
- Analytical & Bioanalytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 164492171
- Full Text :
- https://doi.org/10.1007/s00216-023-04719-2