Understanding and application of Bacillus nitrogen regulation: A synthetic biology perspective.

[Display omitted] • Recent understanding of Bacillus nitrogen metabolism was systematically summarized. • The potential as well as practical applications of nitrogen metabolism regulation in synthetic biology were discussed. • The future research directions of nitrogen metabolism and synthetic biology based on nitrogen metabolism were prospected. • More attention needs to be paid to the role of nitrogen metabolism in improving cell factory performance. Nitrogen sources play an essential role in maintaining the physiological and biochemical activity of bacteria. Nitrogen metabolism, which is the core of microorganism metabolism, makes bacteria able to autonomously respond to different external nitrogen environments by exercising complex internal regulatory networks to help them stay in an ideal state. Although various studies have been put forth to better understand this regulation in Bacillus , and many valuable viewpoints have been obtained, these views need to be presented systematically and their possible applications need to be specified. The intention is to provide a deep and comprehensive understanding of nitrogen metabolism in Bacillus , an important industrial microorganism, and thereby apply this regulatory logic to synthetic biology to improve biosynthesis competitiveness. In addition, the potential researches in the future are also discussed. Understanding the meticulous regulation process of nitrogen metabolism in Bacillus not only could facilitate research on metabolic engineering but also could provide constructive insights and inspiration for studies of other microorganisms. [ABSTRACT FROM AUTHOR]