One step further toward a crop CO2-concentrating mechanism.

The chloroplast envelope transporter LCIA, characterized in the study discussed here, is a bicarbonate channel that facilitates diffusion of bicarbonate into the chloroplast which is driven by the concentration gradient created by active pumping of bicarbonate at the plasma membrane. Keywords: Bicarbonate; carbonic anhydrase; CCM; engineering; photosynthesis; transporter EN Bicarbonate carbonic anhydrase CCM engineering photosynthesis transporter 3402 3405 4 06/30/23 20230627 NES 230627 B The CO b SB B 2 b sb B -concentrating mechanism (CCM) used by eukaryotic algae represents an inorganic carbon pump [Ci: bicarbonate (HCO b SB B 3 b sb SP B - b sp B ), carbon dioxide (CO b SB B 2 b sb B ), and carbonic acid (CO b SB B 3 b sb SP B 2- b sp B )] that generates an elevated concentration of CO b SB B 2 b sb around Rubisco, which promotes carbon fixation. Bicarbonate, carbonic anhydrase, CCM, engineering, photosynthesis, transporter LCIB functions as a carbonic anhydrase: evidence from yeast and Arabidopsis carbonic anhydrase knockout mutants. [Extracted from the article]