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In the mouse cortex, oligodendrocytes regain a plastic capacity, transforming into astrocytes after acute injury.
- Source :
-
Developmental Cell . Jul2023, Vol. 58 Issue 13, p1153-1153. 1p. - Publication Year :
- 2023
-
Abstract
- Acute brain injuries evoke various response cascades directing the formation of the glial scar. Here, we report that acute lesions associated with hemorrhagic injuries trigger a re-programming of oligodendrocytes. Single-cell RNA sequencing highlighted a subpopulation of oligodendrocytes activating astroglial genes after acute brain injuries. By using PLP-DsRed1/GFAP-EGFP and PLP-EGFP mem /GFAP-mRFP1 transgenic mice, we visualized this population of oligodendrocytes that we termed AO cells based on their concomitant activity of a stro- and o ligodendroglial genes. By fate mapping using PLP- and GFAP-split Cre complementation and repeated chronic in vivo imaging with two-photon laser-scanning microscopy, we observed the conversion of oligodendrocytes into astrocytes via the AO cell stage. Such conversion was promoted by local injection of IL-6 and was diminished by IL-6 receptor-neutralizing antibody as well as by inhibiting microglial activation with minocycline. In summary, our findings highlight the plastic potential of oligodendrocytes in acute brain trauma due to microglia-derived IL-6. [Display omitted] • Upon acute brain injuries, oligodendrocytes can activate astroglial genes • Oligodendrocytes can become astrocytes via the transitional AO cell status • Microglia-derived IL-6 drives the transition of oligodendrocytes to AO cells Bai et al. identify a subset of cells in mice with concomitant activation of astro- and oligo-dendroglial genes (termed AO cells), derived from oligodendrocytes upon acute brain injury. AO cells differentiate into astrocytes, oligodendrocytes, and oligodendrocyte precursors, and microglial-derived interleukin-6 promotes the transition of mature oligodendrocytes to AO cells. [ABSTRACT FROM AUTHOR]
- Subjects :
- *OLIGODENDROGLIA
*ASTROCYTES
*BRAIN injuries
*TRANSGENIC mice
*RNA sequencing
Subjects
Details
- Language :
- English
- ISSN :
- 15345807
- Volume :
- 58
- Issue :
- 13
- Database :
- Academic Search Index
- Journal :
- Developmental Cell
- Publication Type :
- Academic Journal
- Accession number :
- 164854758
- Full Text :
- https://doi.org/10.1016/j.devcel.2023.04.016