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Reduced-pressure alkaline pretreatment as an innovative and sustainable technology to extract protein from brewer's spent grain.
- Source :
-
Journal of Cleaner Production . Sep2023, Vol. 416, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
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Abstract
- One of the bottlenecks related to the extraction of protein from lignocellulosic biomass is the development of an efficient, selective, economical, and environmentally friendly process. This study investigated the reduced-pressure alkaline pretreatment (RPAP) as an innovative approach for protein extraction from brewer's spent grain (BSG). Experiments were carried out at different temperatures (30–70 °C), pressures (150–1025 mBar), times (1–7 h), and NaOH concentrations (20–180 mM) to obtain a high-yield and selective protein extraction associated with low CO 2 emissions and high processing capacity per batch. Protein concentration, total CO 2 emissions per protein extracted, selectivity (related to sugars), and processing capacity were the responses considered for optimization. The optimum condition (T = 70 °C; P = 455 mBar, t = 1 h, and [NaOH] = 180.00 mM) was able to extract 80% of the total protein present in BSG composition. This condition was also selective (76.8%) and had a low carbon footprint (0.43 g CO 2 g protein extracted −1). Glutamic acid was the most abundant amino acid in both raw BSG (23.4%) and protein hydrolysate (24.7%), followed by leucine (9.5% and 8.4%, respectively), proline (8.4% and 11.8%, respectively), and aspartic acid (9.0% and 8.1%, respectively). RPAP was demonstrated to be an efficient and sustainable technology for protein extraction from BSG. This opens up new opportunities for BSG and other protein-rich lignocellulosic materials valorization in a biorefinery. [Display omitted] • Brewer's spent grain (BSG) is an important source of protein for food fortification. • Reduced-pressure alkaline pretreatment (RPAP) was efficient to extract protein from BSG. • Optimum RPAP condition extracted protein with high selectivity and low carbon footprint. • Glutamic acid was the most abundant amino acid in BSG and in the protein hydrolysate. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09596526
- Volume :
- 416
- Database :
- Academic Search Index
- Journal :
- Journal of Cleaner Production
- Publication Type :
- Academic Journal
- Accession number :
- 164858743
- Full Text :
- https://doi.org/10.1016/j.jclepro.2023.137966