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Optimization of co-culture condition with respect to aeration and glucose to xylose ratio for bioethanol production.
- Source :
-
Indian Chemical Engineer . Apr2023, Vol. 65 Issue 2, p233-248. 16p. - Publication Year :
- 2023
-
Abstract
- The present study was designed to find a suitable microaerobic condition and ratio of glucose and xylose for maximum ethanol production using co-culture of Saccharomyces cerevisiae and Pichia stipitis. The maximum ethanol concentration and yield were achieved at 0.05 vvm aeration rate and 2:1 glucose/xylose ratio. The co-culture resulted in maximum ethanol concentration, ethanol yield, and volumetric productivity of 12.33 ± 0.10 g/L, 0.43 g/g, and 0.26 g/L/h, respectively. While, the monoculture of P. stipitis resulted in 8.96 ± 0.13 g/L, 0.36 g/g, and 0.19 g/L/h respectively. The fermentation carried out in microaerobic mode delivered 10.68% and 10.56% more ethanol concentration and ethanol yield respectively from glucose compared to the combination of anaerobic and microaerobic mode. Also, the glucose uptake rate increased to 0.83 g/L/h, which corresponds to an improvement of 50.16%, suggesting that the lower microaerophilic condition not only supports P. stipitis metabolism but also does S. cerevisiae to convert glucose faster in a co-culture system. Hence, co-culture cultivation in microaerobic mode would be a better condition to achieve maximum ethanol and productivity. [ABSTRACT FROM AUTHOR]
- Subjects :
- *XYLOSE
*GLUCOSE
*ETHANOL as fuel
*ETHANOL
*SACCHAROMYCES cerevisiae
*PICHIA
Subjects
Details
- Language :
- English
- ISSN :
- 00194506
- Volume :
- 65
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Indian Chemical Engineer
- Publication Type :
- Academic Journal
- Accession number :
- 164872033
- Full Text :
- https://doi.org/10.1080/00194506.2023.2190332