Measurement of amyloid-β 1–42 in cerebrospinal fluid: a comparison of the second generation Elecsys and INNOTEST.

Finally, because the Elecsys version 1 preanalytical protocol recommended sample storage in -80 °C, we also compared the concentration measurements of A 42 after a single freeze-thaw cycle, using both Sarstedt V1 (25 samples) and Sarstedt V2 (21 samples). Keywords: Alzheimer's dementia; amyloid; biomarkers; Elecsys EN Alzheimer's dementia amyloid biomarkers Elecsys e182 e185 4 07/18/23 20230801 NES 230801 To the Editor, Amyloid-beta 1-42 (A 42) is one of the diagnostic cerebrospinal fluid biomarkers for Alzheimer's dementia [[1]], together with Tau proteins. For Elecsys version 2, we applied the recommended (by Roche) cutoff of 1,030 ng/L. However, in the case of INNOTEST, an upwards drift in the has been observed for results of A 42 within the last 10 years, with data suggesting that the optimal cutoff for INNOTEST A 42 is not constant, but rather period-dependent [[4]], [[5]], [[6]]. [Extracted from the article]