A near-infrared fluorescence-on fluorescent probe for formaldehyde imaging in Arabidopsis thaliana.

As a reactive carbonyl molecule, formaldehyde (FA) plays an important part in plant physiological and pathological activities. It is necessary to probe FA in plant for the study of its functions. In this paper, we report DISE , a dicyanoisophorone-based turn-on near-infrared fluorescent probe, which exhibits a highly sensitive response to FA. In addition, confocal imaging experiments showed that DISE could image endogenous and exogenous FA in Arabidopsis root tissue. It demonstrates that the probe has the potential to be an imaging tool to research the FA's biological roles in plant. [Display omitted] • A new near-infrared fluorescence probe (DISE) towards to formaldehyde (FA) is reported. • DISE can selectively detect FA with a large Stokes shift and a large high emission enhancement factor. • DISE can monitor exogenous and endogenous FA in live Arabidopsis thaliana root tissues. [ABSTRACT FROM AUTHOR]