Synergistic analysis of performance, microbial community, and metabolism in aerobic granular sludge under polyacrylonitrile microplastics stress.

[Display omitted] • PAN MPs enriched the secretion of extracellular polymeric substance. • Granular breakage occurred at 100 mg/L PAN MPs. • Phylum Proteobacteria played a dominant role in resistance to PAN MPs. • PAN MPs inhibited membrane transport and denitrification functional genes. Aerobic granular sludge (AGS) has proved to be a promising biotechnology for microplastics wastewater treatment. However, polyacrylonitrile microplastics (PAN MPs), the most widely used plastic in textile materials, have not been investigated. Therefore, the effect of the neglected PAN MPs on AGS at different concentrations (1, 10, and 100 mg/L) was evaluated. The results indicated that PAN MPs with 1 and 10 mg/L concentrations had no obvious effect on granular stability and nutrient removal performance, but greatly promoted the secretion of EPS. Remarkably, the granule structure was severely damaged under 100 mg/L PAN MPs. Moreover, microbial community analysis showed that phylum Proteobacteria played a dominant role in resistance to PAN MPs. Metabolic analysis further revealed that genes related to denitrification pathway (nasA, nirK, nirS and norB) and membrane transport were significantly inhibited under PAN MPs stress. This study may provide additional information on the treatment of microplastics wastewater using AGS. [ABSTRACT FROM AUTHOR]