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Modulation of inner junction proteins contributes to axoneme differentiation.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America . 7/25/2023, Vol. 120 Issue 30, Following p1-11. 24p. - Publication Year :
- 2023
-
Abstract
- Cilia build distinct subdomains with variable axonemal structures to perform diverse functions in cell motility and signaling. In sensory cilia across species, an axoneme differentiates longitudinally into a middle segment with nine microtubule (MT) doublets and a distal segment with nine MT singlets that extends from the A tubules of the doublets. Here, we study axoneme differentiation in Caenorhabditis elegans by analyzing the flagellar inner junction protein FAP20 and PCRG1 that connect A and B tubules in Chlamydomonas. The nematode CFAP-20 is restricted to the middle segment with doublets, and its loss disconnects A and B tubules. However, PCRG-1 is absent from most sensory cilia, and its deletion does not disrupt cilia. Ectopic introduction of PCRG-1 into cilia generated abnormal MT doublets in the distal segment and reduced intraflagellar transport and animal sensation. Thus, the absence of an inner junction protein prevents B-tubule extension, which contributes to axoneme differentiation and ciliary function. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00278424
- Volume :
- 120
- Issue :
- 30
- Database :
- Academic Search Index
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 165561972
- Full Text :
- https://doi.org/10.1073/pnas.2303955120