中药抑制间充质干细胞衰老的作用及机制.

BACKGROUND: The aging of mesenchymal stem cells is one of the main causes of aging-related diseases, and seriously affects its clinical application. Traditional Chinese medicine has a good anti-aging effect, and it can inhibit the aging of mesenchymal stem cells to promote its application in tissue engineering and prevent and treat aging-related diseases. OBJECTIVE: To review the effect and mechanism of traditional Chinese medicine on inhibiting the aging of mesenchymal stem cells. METHODS: We searched CNKI and PubMed for the literature on inhibiting mesenchymal stem cell aging with traditional Chinese medicine from 2012 to 2022. The keywords were “traditional Chinese medicine, mesenchymal stem cells (MSCs), aging” in Chinese and English, respectively. Finally, 92 articles were included for further review. RESULTS AND CONCLUSION: (1) We summarized five main mechanisms of the aging of mesenchymal stem cells: DNA damage, telomere shortening, oxidative stress, autophagy disorder and mitochondrial dysfunction. (2) This paper reviewed the phenotypic characteristics of senescent mesenchymal stem cells, including increases in cell volume, decreases in proliferation and multi-directional differentiation, increases in β-galactosidase activity, and activation of p21 and p16 pathways, and so on. (3) We summarized the main mechanisms of Chinese medicine inhibiting the senescence of mesenchymal stem cells at present, including inhibiting the activation of the Wnt pathway, inhibiting the production of mitochondrial reactive oxygen species, promoting the silencing of information regulator factor 2 homolog 1, phosphatidylinositol 3-kinase/protein kinase B, adenosine 5’-monophosphate-activated protein kinase and nuclear factor E2 related factor 2 pathway activation, and promoting the expression of telomerase reverse transcriptase. (4) At present, bone marrow mesenchymal stem cells are the most widely studied in the research of traditional Chinese medicine to inhibit the aging of bone marrow mesenchymal stem cells, and the effect is better. (5) Zuogui Wan, Bushen Tiaogan Formula, resveratrol, Astragalus membranaceus and other traditional Chinese medicines can prevent and treat osteoporosis by promoting the proliferation and osteogenic differentiation of aging mesenchymal stem cells. However, the mechanism of Chinese medicine in improving the paracrine function of mesenchymal stem cells and preventing other aging-related diseases by inhibiting the aging of mesenchymal stem cells needs to be further explored. [ABSTRACT FROM AUTHOR]