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Paper-based biosensors relying on core biological immune scaffolds for the detection of procymidone in vegetables.

Authors :
Li, Gaozhen
Sun, Jiashuai
Li, Jiahuan
Zhang, Yaoli
Huang, Jingcheng
Yue, Fengling
Dong, Haowei
Li, Falan
Xu, Huihui
Guo, Yanyin
Guo, Yemin
Sun, Xia
Source :
Talanta. Dec2023, Vol. 265, pN.PAG-N.PAG. 1p.
Publication Year :
2023

Abstract

In order to achieve a highly sensitive detection of procymidone in vegetables, three paper-based biosensors based on a core biological immune scaffold (CBIS) were developed, which were time-resolved fluorescence immunochromatography strips with Europium (III) oxide (Eu-TRFICS). Goat anti-mouse IgG and europium oxide time-resolved fluorescent microspheres formed secondary fluorescent probes. CBIS was formed by secondary fluorescent probes and procymidone monoclonal antibody (PCM-Ab). The first type of Eu-TRFICS (Eu-TRFICS-(1)) fixed secondary fluorescent probes on a conjugate pad, and PCM-Ab was mixed with a sample solution. The second type of Eu-TRFICS (Eu-TRFICS-(2)) fixed CBIS on the conjugate pad. The third type of Eu-TRFICS (Eu-TRFICS-(3)) was directly mixed CBIS with the sample solution. They solved the problems of steric hindrance of antibody labeling, insufficient exposure of antigen recognition region and easy loss of activity in traditional methods. They realized multi-dimensional labeling and directional coupling. They replaced the loss of antibody activity. And the three types of Eu-TRFICS were compared, among which Eu-TRFICS-(1) was the best detection choice. Antibody usage was reduced by 25% and sensitivity was increased by 3 times. Its detection range was 1–800 ng/mL, the limit of detection (LOD) was 0.12 ng/mL with the visible LOD (vLOD) of 5 ng/mL. [Display omitted] • Time-resolved fluorescent microspheres were applied to CBIS. • The monoclonal antibody usage was reduced by 25% prepared by the CBIS. • The CBIS connect multiple microspheres to one antibody molecule. • The linear range of Eu-TRFICS-(1) used to detect procymidone is 1–800 ng/mL. • Three kinds of Eu-TRFICS used to detect procymidone are constructed and compared. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00399140
Volume :
265
Database :
Academic Search Index
Journal :
Talanta
Publication Type :
Academic Journal
Accession number :
168587800
Full Text :
https://doi.org/10.1016/j.talanta.2023.124843