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The anti-cell death FNK protein protects cells from death induced by freezing and thawing
- Source :
-
Biochemical & Biophysical Research Communications . May2005, Vol. 330 Issue 3, p850-856. 7p. - Publication Year :
- 2005
-
Abstract
- Abstract: The FNK protein, constructed from anti-apoptotic Bcl-xL with enhanced activity, was fused with the protein transduction domain (PTD) of the HIV/Tat protein to mediate the delivery of FNK into cells. The fusion protein PTD-FNK was introduced into chondrocytes in isolated articular cartilage-bone sections, cultured neurons, and isolated bone marrow mononuclear cells to evaluate its ability to prevent cell death induced by freezing and thawing. PTD-FNK protected the cells from freeze–thaw damage in a concentration-dependent manner. Addition of PTD-FNK with conventional cryoprotectants (dimethyl sulfoxide and hydroxyethyl starch) increased surviving cell numbers around 2-fold compared with controls treated only with the cryoprotectants. Notably, PTD-FNK allowed CD34+ cells among bone marrow mononuclear cells to survive more efficiently (12-fold more than the control cells) from two successive freeze–thaw cycles. Thus, PTD-FNK prevented cell death induced by freezing and thawing, suggesting that it provides for the successful cryopreservation of biological materials. [Copyright &y& Elsevier]
- Subjects :
- *BONE marrow
*IMMUNE system
*MICROBIAL genetics
*CONNECTIVE tissues
Subjects
Details
- Language :
- English
- ISSN :
- 0006291X
- Volume :
- 330
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Biochemical & Biophysical Research Communications
- Publication Type :
- Academic Journal
- Accession number :
- 16874348
- Full Text :
- https://doi.org/10.1016/j.bbrc.2005.03.059