Potent inhibition of chitin synthase by an azasugar—investigation of synergistic effect with UDP.

We identified 6-deoxy-homoDMDP as a potent inhibitor of chitin synthase (Ki=38?µM), displaying an uncompetitive inhibition pattern. Dual inhibition was also performed with the enzymatic reaction product uridine 5'-diphosphate in order to evaluate the concurrent effect of both inhibitors. An interaction coefficient a of 0.9 was found, revealing synergistic inhibition. [ABSTRACT FROM AUTHOR]