Optimization of extraction of Selenium enriched passion fruit seed oil with supercritical CO2 extraction technology.

Selenium enriched passion fruit seed oil was prepared by supercritical CO2 extraction method with Selenium enriched passion fruit seed as raw material. Based on single factor experiment, RSM-CCD ( response surface curve-central composite design) was used to explore the effects of temperature, pressure, static extraction time, dynamic extraction time and CO2 flow rate on the extraction rate of Selenium enriched passion fruit seed oil. And the physical and chemical properties, fatty acid content and antioxidant activity of selenium-rich passionfruit seed oil extracted by supercritical CO2 extraction and Soxhlet extraction were investigated. The results showed that optimal process parameters of supercritical CO2 extraction for oil from Selenium enriched passion seed was found to be extraction temperature of 50 °C,extraction pressure of 34 MPa, static extraction of 109 min,dynamic extraction of 97 min, CO2 flow rate of 1.0 L/min. Under this condition, the actual Selenium enriched passion seed oil was clear light yellow liquid, and the yield was 72. 33%. The content of unsaturated fatty acids extracted by supercritical CO2 extraction was 80. 11%, which was slightly higher than that extracted by soxhlet extraction (78.71%); Acid value, peroxide value, oxidation resistance and other properties also showed that the quality of oil extracted by supercritical CO2 extraction was better than that extracted by Soxhlet extraction. [ABSTRACT FROM AUTHOR]