Neodymium affects the generation of reactive oxygen species via GSK-3β/Nrf2 signaling in the gill of zebrafish.

• Neodymium decreases the activity and gene expression of antioxidant enzymes. • Neodymium increases the generation of ROS in the gill of zebrafish. • Neodymium inhibits GSK-3β/Nrf2 signaling in the gill of zebrafish. • Neodymium affects the generation of ROS via GSK-3β/Nrf2 signaling. Rare earth element neodymium (Nd) is widely used in industry and agriculture, which may result in the pollution of aquatic environment. In this study, we exposed zebrafish with 10, 50, and 100 μg/L Nd for four weeks. The results showed that Nd could be accumulated in fish gill and Nd accumulation affected the equilibrium of nutrient elements. Nd decreased the antioxidant enzymes' activity and gene expression level, but enhanced the generation of reactive oxygen species (ROS). Moreover, various concentration of Nd treatments inhibited Nrf2 signaling in gill. To examine the critical role of GSK-3β/Nrf2 signaling on ROS generation under Nd stress, we further interfered gsk-3β gene in zebrafish under 100 μg/L Nd exposure. The result showed that gsk-3β gene interference induced Nrf2 signaling as well as the expression and activity of antioxidant enzymes in fish gill. In all, Nd could be accumulated in fish gill, and the signaling of GSK-3β/Nrf2 was involved in regulating ROS generation under Nd treatments. [ABSTRACT FROM AUTHOR]