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Dual-mode sensing platform based on aptamer-tunable catalytic activity of mesoporous polydopamine/MnO2 nanozymes for detecting S. aureus.
- Source :
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Sensors & Actuators B: Chemical . Oct2023, Vol. 393, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Staphylococcus aureus (S. aureus) infections pose a serious threat to human health worldwide. Therefore, selective and accurate detection of S. aureus is necessary for preventing infections. Herein, we design the colorimetric-electrochemical sensing platform based on specific oligonucleotide (SA31 aptamer)-tunable oxidase-like activity of mesoporous polydopamine/MnO 2 (MPDA/MnO 2) nanozymes for the S. aureus detection. The MPDA/MnO 2 nanozymes possess excellent oxidase-like activity and electrochemical property. MPDA/MnO 2 nanozymes can catalyze the oxidation of TMB to generate TMB+ (blue color) and TMB2+ (yellow color) products. The oxidase-like activity of MPDA/MnO 2 can be inhibited when the SA31 is adsorbed on the surface of the nanozymes. In the presence of S. aureus, SA31 aptamer desorbs from MPDA/MnO 2 surface based on the specific identification between S. aureus and aptamer, resulting in the recovery of their oxidase-like activity. Based on the different oxidative degree of TMB, the color of sensing solution changes from blue to yellow, the electrochemical signals also have changed simultaneously. The colorimetric-electrochemical sensing platform can accurately detect S. aureus, showing a low detection limit in 3 CFU/mL (S/N = 3). Furthermore, the dual-mode sensing platform can be utilized for the accurate and specific detection of S. aureus in real sample with the recovery of 95.15%− 115.28%. This work based on SA31 aptamer-tunable oxidase-like activity of MPDA/MnO 2 nanozymes provides a dual-mode assay for specific and accurate detection of S. aureus. • A dual-modal strategy was designed to detect S. aureus based on the colorimetric-electrochemical sensing platform. • The SA31 can regulate the oxidase-like activity by adsorption and desorption on the surface of MPDA/MnO 2 nanozymes. • The electrochemical behavior of the electrode can be adjusted by the specific binding between aptamers and S. aureus. • This dual-modal sensing platform possesses low detection limit (3 CFU/mL) and wide linear range. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09254005
- Volume :
- 393
- Database :
- Academic Search Index
- Journal :
- Sensors & Actuators B: Chemical
- Publication Type :
- Academic Journal
- Accession number :
- 169968336
- Full Text :
- https://doi.org/10.1016/j.snb.2023.134218