胸腺素 β4 在乳腺癌组织中的表达及其对乳腺癌细胞迁移和 侵袭的影响.

Objective: To investigate the expression of thymosin beta 4 (Tβ4) in the breast cancer tissue and its effect on the migration and invasion of the breast cancer MCF-7 cells, and to clarify its possible mechanism. Methods: Immunohistochemical staining was used to detect the expressions of Tβ4 protein in cancer tissue of 67 female breast cancer patients. The human breast cancer MCF-7 cells were divided into blank control group, siRNA-control group, and siRNA-Tβ4 group. The expression levels of Tβ4 mRNA in the cells in various groups were detected by real-time fluorescence quantitative PCR (RT-qPCR) method, the numbers of invasion cells in various groups were detected by Transwell chamber assay, and the scratch healing rates of cells in various groups were detected by cell scratch assay. Results: Among 67 breast cancer patients, the Tβ4 was positively expressed in tumor tissue of 59 patients (88. 06%). The positive expression rates of Tβ4 was associated with the T stage, N stage, and clinical stage of the breast cancer patients (P<0. 05); there were no significant correlations between the positive expression rate of Tβ4 with patients’ age, estrogen receptor (ER) expression, human epidermal growth factor receptor-2 (HER2) expression, and Ki-67 expression (P>0. 05). Compared with blank control group and siRNA-control group, the expression level of Tβ4 mRNA in the cells in siRNA-Tβ4 group was decreased (P<0. 01), the number of invasion cells was decreased (P<0. 05), and the scratch healing rate of the cells was significantly decreased (P<0. 01). Conclusion: The expression level of Tβ4 in breast cancer cells is high, the expression of Tβ4 is closely correlated with the T stage, N stage and clinical stage, and targeted silencing Tβ4 could inhibit the invasion and migration abilities of the breast cancer cells. [ABSTRACT FROM AUTHOR]