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Evaluating dynamic traveling wave profiles for the enhancement of separation and sensitivity in traveling wave structures for lossless ion manipulations.

Authors :
Kinlein, Zackary
Clowers, Brian H.
Source :
Journal of Chromatography A. Sep2023, Vol. 1706, pN.PAG-N.PAG. 1p.
Publication Year :
2023

Abstract

• The performance of multiple dynamic traveling wave profiles was evaluated. • Optimal resolution was obtained using a static TW, but the ramp performed similarly. • Regarding SNR, optimum waveform profiles were species dependent. • Increases in ion abundance were observed when using a ramped TW for select species. • Tailored waveforms to optimize TW-SLIM separations require continued refinement. The amenability of traveling wave ion mobility spectrometry (TWIMS) to extended separation pathlengths has prompted a recent surge of interest concerning the technique. While promising, the optimization of ion transmission, particularly when analyzing increasingly disparate species, remains an obstacle in TWIMS. To address this issue, we evaluated a suite of dynamic TW profiles using an original TW structures for lossless ion manipulations (TW-SLIM) platform developed at Washington State University. Inspired by the range of gradient elution profiles used in traditional chromatography, three distinct square TW profiles were evaluated: a static approach which represents a traditional waveform, a dual approach which consists of two distinct TW profiles within a given separation event; and a ramp approach which varies TW speed and amplitude at a fixed rate during separation. The three waveform profiles were evaluated in terms of their impact on separation (quantified as resolution) and sensitivity (quantified using signal-to-noise ratio (SNR), and ion abundance). Concerning separation, the highest resolution (R) was observed when operating with the static waveform (R = 7.92); however, the ramp waveform performed comparably (R = 7.70) under similar conditions. Regarding SNR, optimum waveform profiles were species dependent. Bradykinin2+ displayed the largest gains in SNR (36.6% increase) when ramping TW speed, while the gains were greatest (33.5% increase) for tetraoctylammonium when modulating TW amplitude with the static waveform. Lastly, significant (>10%) increases in the abundance of tetraoctylammonium ions were observed exclusively when utilizing a ramped waveform. The present set of experiments outline the results and challenges related to optimizing separations using alternative TW profiles and provides insight concerning TW-SLIM method development which may be tailored to enhance select analytical metrics. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00219673
Volume :
1706
Database :
Academic Search Index
Journal :
Journal of Chromatography A
Publication Type :
Academic Journal
Accession number :
170067081
Full Text :
https://doi.org/10.1016/j.chroma.2023.464207