海拔高度对会理烤烟品质及代谢组学的影响.

【Objective】 The present paper aimed to explore the relationship between altitude and flue-cured tobacco quality, style and metabolites in Huili.【Method】 Using Yunyan87 as the research material, the effects of different altitudes in the range of 1550-2300 meters in the Huili tobacco area region on the conventional chemical composition, sensory quality, and metabolomics of tobacco were studied.【Result】 A higher altitude of tobacco planting was more conducive to the accumulation of total sugars, reducing sugars, and petroleum ether extracts in tobacco, but the content of nicotine and total nitrogen was opposite. There was a significant and strong positive correlation between sugar-alkali ratio and altitude. With the increase of altitude, the aroma quality of flue-cured tobacco was improved, the aroma quantity was increased, the offensive odor was reduced, and the style sweet flavor was more typical, but the impact decreased. A total of 28 different metabolites were screened by the OPLS-DA model, and the nicotine of flue-cured tobacco at low altitudes and the niacin and mesmine, which were closely related to it, were higher expressed. The organic acid substances such as nonanoic acid and hexanoic acid had the richest content in tobacco from mid-altitude areas. Relative content of alcohol derivatives such as benzyl alcohol, phenylethanol, maltol and degradation product of plastid pigment such as neophytadiene, damascene 2-buten-1-one, β-ionone, 2 (4h) -benzofuranone of flue-cured tobacco in high altitude area was the highest; The different metabolites were annotated to six B-level metabolic pathways, including carbohydrate metabolism, amino acid metabolism, terpenoid and polyketone compound metabolism, and so on. Among them, altitude had the most significant impact on tobacco leaf carbohydrate metabolism and amino acid metabolism. 【Conclusion】 The increase in total sugars, reducing sugars, sugar-alkali ratio, alcohol derivatives and degradation product of plastid pigment is the important reasons for the improvement of the sensory quality and the more prominent style characteristics of flue-cured tobacco in high-altitude areas. Therefore, an appropriate increase in the altitude of tobacco planting in the Huili tobacco area is conducive to improving the quality of the flue-cured tobacco in the producing area and its sweet fragrance style. [ABSTRACT FROM AUTHOR]