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Soil organic matter and nutrient availability affect the applicability of low-carbon energy source in Dehalococcoides-augmented soil.
- Source :
-
Journal of Hazardous Materials . Oct2023, Vol. 459, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Dehalococcoides is a functional microorganism that completely dechlorinates trichloroethene (TCE). Augmentation with pure Dehalococcoides is important for reducing environmental disturbances that accompany bioaugmentation. However, the applicability of Dehalococcoides -bioaugmentation to contaminated soils is unclear. In this study, seven low-carbon energy sources (methanol, formate, acetate, ethanol, lactate, citrate, and benzoate) were used as electron donors for Dehalococcides to evaluate its applicability in remediating TCE-contaminated soils. Soil microcosms supplemented with ethanol, formate, or lactate showed relatively high dechlorination activity within 111–180 days. The functional gene profiles predicted by PICRUSt2 from 16 S rRNA gene sequences were similar in the proportions of dehydrogenases, which initiate electron donor oxidation, in all soils and did not seem to reflect Dehalococcoides -bioaugmentation applicability. Soils with higher organic matter content (>3.2%; dry weight base) and protein concentration (>1.6 µg/mL) supported complete dechlorination. These results suggest that organic matter and nutrient availability mainly affect successful TCE dechlorination in Dehalococcoides -augmented soils. The study offers significant experimental support for comprehending the suitability of low-carbon energy sources in successful bioaugmentation, aiming to mitigate environmental disturbances associated with the process. [Display omitted] • Ethanol, formate, and lactate are low-carbon energy sources for effective dechlorination. • Formate supplementation achieved the fastest and most complete dechlorination within 111–180 days. • Soils with higher organic matter content and protein concentration supported complete dechlorination. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03043894
- Volume :
- 459
- Database :
- Academic Search Index
- Journal :
- Journal of Hazardous Materials
- Publication Type :
- Academic Journal
- Accession number :
- 170720896
- Full Text :
- https://doi.org/10.1016/j.jhazmat.2023.132251