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PABPN1 aggregation is driven by Ala expansion and poly(A)-RNA binding, leading to CFIm25 sequestration that impairs alternative polyadenylation.
- Source :
-
Journal of Biological Chemistry . Aug2023, Vol. 299 Issue 8, p1-17. 17p. - Publication Year :
- 2023
-
Abstract
- Poly(A)-binding protein nuclear 1 (PABPN1) is an RNA- binding protein localized in nuclear speckles, while its alanine (Ala)-expanded variants accumulate as intranuclear aggregates in oculopharyngeal muscular dystrophy. The factors that drive PABPN1 aggregation and its cellular consequences remain largely unknown. Here, we investigated the roles of Ala stretch and poly(A) RNA in the phase transition of PABPN1 using biochem- ical and molecular cell biology methods. We have revealed that the Ala stretch controls its mobility in nuclear speckles, and Ala expansion leads to aggregation from the dynamic speckles. Poly(A) nucleotide is essential to the early-stage condensation that thereby facilitates speckle formation and transition to solid- like aggregates. Moreover, the PABPN1 aggregates can sequester CFIm25, a component of the pre-mRNA 30-UTR processing complex, in an mRNA-dependent manner and consequently impair the function of CFIm25 in alternative polyadenylation. In conclusion, our study elucidates a molecular mechanism under- lying PABPN1 aggregation and sequestration, which will be beneficial for understanding PABPN1 proteinopathy. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00219258
- Volume :
- 299
- Issue :
- 8
- Database :
- Academic Search Index
- Journal :
- Journal of Biological Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 170726980
- Full Text :
- https://doi.org/10.1016/j.jbc.2023.105019