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In Vivo and In Vitro Silica Induces Nuclear Factor Egr-1 Activation Mediated by ERK 1/2 in RAW264.7 Cell Line.
- Source :
-
Toxicology Mechanisms & Methods . Mar/Apr2005, Vol. 15 Issue 2, p93-99. 7p. - Publication Year :
- 2005
-
Abstract
- The transcription factor early growth response gene (Egr-1) is a stress response gene activated by various forms of stress. The effect of silica on transcription and expression of Egr-1 was investigated in rat lung and in RAW264.7 cells. Silica induced the expression of Egr-1 in vivo and was mainly located in alveolar macrophage cells and lung epithelial cells. Furthermore, silica induced Egr-1 mRNA and protein expression in cultured RAW264.7 cells. Immunofluorescence microscopy revealed translocation of Egr-1 to the nucleus in response to silica. The contribution of the extracellular signal-regulated kinase (ERK) pathway to the activation of Egr-1 in response to silica was examined. Exposure to silica resulted in a rapid phosphorylation of ERK 1/2 kinases in RAW264.7 cells. MAP Kinase Kinase (MEK) inhibitor U0126 prevented Egr-1 induction by silica. The results suggest that silica could induce Egr-1 activation in macrophages in vivo and in vitro and that phosphorylated ERK 1/2 may be involved in this action. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15376516
- Volume :
- 15
- Issue :
- 2
- Database :
- Academic Search Index
- Journal :
- Toxicology Mechanisms & Methods
- Publication Type :
- Academic Journal
- Accession number :
- 17121650
- Full Text :
- https://doi.org/10.1080/15376520590918775