Cascade dynamic assembly/disassembly of DNA nanoframework enabling the controlled delivery of CRISPR-Cas9 system.

The article discusses the development of a DNA nanoframework (NF) that utilizes dynamic assembly/disassembly to control the delivery of Cas9 ribonucleoprotein (RNP) for gene editing. This strategy involves the incorporation of sgRNA into the NF through hybridization chain reaction (HCR), followed by the loading of Cas9 protein into the expanded internal space of the NF.