Synergistic catalysis and detection of hydrogen peroxide based on a 3D-dimensional molybdenum disulfide interspersed carbon nanotubes nanonetwork immobilized chloroperoxidase biosensor.

[Display omitted] • An efficient biosensor 3D-CNT@MoS 2 /IL EMB /CPO for H 2 O 2 detection. • 3D-CNT@MoS 2 increases the fixation site of the CPO while prevents it leaking. • CPO achieves direct electron transfer in the absence of a redox medium. • The biosensor is applied to the detection of H 2 O 2 in human urine. Enzyme-based electrochemical biosensors are promising for a wide range of applications due to their unique specificity and high sensitivity. In this work, we present a novel enzyme bioelectrode for the sensing of hydrogen peroxide (H 2 O 2). The molybdenum disulfide nanoflowers (MoS 2) is self-assembled on carboxylated carbon nanotubes (CNT) to form a three-dimensional conductive network (3D-CNT@MoS 2), which is modified with 1-ethyl-3-methylimidazolium bromide (IL EMB), and followed by anchoring chloroperoxidase (CPO) onto the nanocomposite (3D-CNT@MoS 2 /IL EMB) through covalent binding to form a bioconjugate (3D-CNT@MoS 2 /IL EMB /CPO). The IL EMB modified 3D-CNT@MoS 2 /IL EMB has good hydrophilicity and conductivity, which not only provides a suitable microenvironment for the immobilization of CPO but also facilitates the direct electron transfer (DET) of CPO at the electrode. The 3D-CNT@MoS 2 /IL EMB /CPO bioconjugate modified electrode has a high catalytic efficiency for H 2 O 2. Through electroenzymatic synergistic catalysis for H 2 O 2 detection by 3D-CNT@MoS 2 /IL EMB /CPO-GCE, a wide detection range of 0.2 μmol·L−1 to 997 μmol·L−1 and a low detection limit of 0.097 μmol･L−1 with high sensitivity of 1050 µA·mmol·L−1·cm−2 were achieved. Additionally, the 3D-CNT@MoS 2 /IL EMB /CPO-GCE displayed exceptional stability, selectivity, and reproducibility. Furthermore, 3D-CNT@MoS 2 /IL EMB /CPO-GCE is suitable for sensing of H 2 O 2 in human urine s with good recovery, suggesting its potential application for the detection of H 2 O 2 in biomedical field. [ABSTRACT FROM AUTHOR]