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Development of recombinant sialidase (NanH) protein-based Indirect-ELISA for epidemiological survey of anti-Pasteurella multocida antibodies in bovines.
- Source :
-
Indian Journal of Experimental Biology . Sep2023, Vol. 61 Issue 9, p697-704. 8p. - Publication Year :
- 2023
-
Abstract
- Haemorrhagic septicaemia (HS) is a highly contagious and fatal disease of cattle and buffaloes and causes major economic losses to farmers. Though indirect hemagglutination (IHA) test has been used to detect a specific antibody against P. multocida, it has low specificity for sero-diagnosis of HS. Therefore, development of a rapid, highly sensitive and specific serological test is a prerequisite for detection of antibodies against HS. In this context, we explored an in-house ELISA method using recombinant antigens for detection of antibodies against P. multocida in bovines. nanH gene from P. multocida B:2 strain P52 was cloned and the recombinant mature protein with a C- and N-terminal truncation was produced as a fusion protein (~63 kDa) in Escherichia coli. The immunogenic potential of purified rNanH-Tr was assessed by the Western blot method using specific anti-rNanH-Tr antibody responses in sera collected from immunized rabbits. An indirect-ELISA based on rNanH-Tr was developed and optimized. Furthermore, the rNanH-Tr ELISA was applied to screen bovine serum samples (n=250). The receiver operating characteristic curve analysis for the detection of anti P. multocida specific antibodies indicated a diagnostic sensitivity of 86.2 (CI 73.26-96.80%) and specificity of 80.0 (63.06-91.56%). No cross reactivity was noted with antibodies against other bovine diseases (e.g., foot-and-mouth disease and brucellosis). Screening of random bovine serum samples showed a 22% sero-positivity for anti P. multocida specific antibodies. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00195189
- Volume :
- 61
- Issue :
- 9
- Database :
- Academic Search Index
- Journal :
- Indian Journal of Experimental Biology
- Publication Type :
- Academic Journal
- Accession number :
- 171933104
- Full Text :
- https://doi.org/10.56042/ijeb.v61i09.3872