A disposable non-enzymatic dual sensor for simultaneous amperometric determination of NADH and H2O2.

The determination of nicotinamide adenine dinucleotide (NADH) and hydrogen peroxide (H2O2) play critical roles in chemical, environmental, and biological processes. In this study, non-enzymatic dual sensors for the simultaneous amperometric determination of NADH and H2O2 were prepared based on a dual screen-printed carbon electrode (DSPCE) modified with reduced graphene oxide (RGO), polyneutral red (PNR), and gold nanoparticles (AuNP). The DSPCE/RGO/PNR/AuNP electrode was characterized by cyclic voltammetry and scanning electron microscopy. RGO/PNR/AuNP materials showed an electrocatalytic effect on the electrooxidation of NADH and the electroreduction of H2O2. Simultaneous amperometric determination of NADH and H2O2 was performed at + 0.15 V and − 0.45 V, respectively. The developed dual sensor allowed the simultaneous determination of both analytes in fast, high repeatability and sensitivity (2.28 µA mM−1 for NADH and 2.70 µA mM−1 for H2O2) and a low limit of detection (5.53 µA for NADH and 8.35 µA for H2O2). The application stability of the dual sensor was tested for 45 days. The simultaneous determination of NADH and H2O2 in blood serum was performed using the fabricated non-enzymatic dual sensor, and high recoveries were obtained. The developed disposable dual sensors can be used in point-of-care tests for simultaneous amperometric determination of NADH and H2O2. A disposable non-enzymatic dual sensor for simultaneous amperometric determination of NADH and H2O2 [ABSTRACT FROM AUTHOR]