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Construction of an ATP-Activated Y-Shape DNA Probe for Smart miRNA Imaging in Living Cells.
- Source :
-
Chemistry (2624-8549) . Sep2023, Vol. 5 Issue 3, p1634-1644. 11p. - Publication Year :
- 2023
-
Abstract
- A stringent DNA probe to profile microRNA (miRNA) expression within a specific cell remains a key challenge in biology. To address this issue, an intracellular ATP-activated Y-DNA probe for accurate imaging of miRNA in living cells was designed. Y-DNA was based on the fabrication of tripartite function modules, which consisted of a folate (FA)-modified targeting module, an ATP aptamer-sealed driver, and a miRNA sensing module. The Y-DNA probe could be specifically activated by ATP after it efficiently internalized into FA-receptor-overexpressed cells based on caveolar-mediated endocytosis, leading to the activation of the miRNA sensing module. The activated Y-DNA probe allowed for the imaging of miRNA in living cells with high sensitivity. The design of the ATP-activated Y-DNA sensor opens the door for bioorthogonal miRNA imaging and promotes the development of various responsive DNA molecular probes with enhanced anti-interference ability for clinical diagnosis. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 26248549
- Volume :
- 5
- Issue :
- 3
- Database :
- Academic Search Index
- Journal :
- Chemistry (2624-8549)
- Publication Type :
- Academic Journal
- Accession number :
- 172393738
- Full Text :
- https://doi.org/10.3390/chemistry5030112