Asarinin, a furofuran lignan compound isolated from Horsfieldia spicata.

Our continuous investigation of the chemical constituent of the leaves of Horsfieldia spicata (Myristicaceae) led to the isolation of asarinin, a furofuran lignan compound. H. spicata dried leaves were macerated with methanol to obtained methanol extract (ME), ME further fractionated with n-hexane (HF), ethyl acetate (EAF) and butanol (BF). EAF subjected to column chromatography (CC)(silica gel 0.2-0.5 mm, gradient n-hexane/ethyl acetate/methanol with 5% increase in polarity), a white needle crystal successfully isolated from sub-fraction of HDEA-SF 2. The chemical structure of asarinin was elucidated based on spectroscopic data collected from LC-MS and NMR data (1D-and 2D-NMR). [ABSTRACT FROM AUTHOR]