Combination of transcriptomic, proteomic, and degradomic profiling reveals common and distinct patterns of pathogen‐induced cell death in maize.

SUMMARY: Regulated cell death (RCD) is crucial for plant development, as well as in decision‐making in plant‐microbe interactions. Previous studies revealed components of the molecular network controlling RCD, including different proteases. However, the identity, the proteolytic network as well as molecular components involved in the initiation and execution of distinct plant RCD processes, still remain largely elusive. In this study, we analyzed the transcriptome, proteome, and N‐terminome of Zea mays leaves treated with the Xanthomonas effector avrRxo1, the mycotoxin Fumonisin B1 (FB1), or the phytohormone salicylic acid (SA) to dissect plant cellular processes related to cell death and plant immunity. We found highly distinct and time‐dependent biological processes being activated on transcriptional and proteome levels in response to avrRxo1, FB1, and SA. Correlation analysis of the transcriptome and proteome identified general, as well as trigger‐specific markers for cell death in Zea mays. We found that proteases, particularly papain‐like cysteine proteases, are specifically regulated during RCD. Collectively, this study characterizes distinct RCD responses in Z. mays and provides a framework for the mechanistic exploration of components involved in the initiation and execution of cell death. Significance Statement: This work combines a large‐scale transcriptomic, proteomic, and N‐terminomic analysis of maize leaves to obtain insight into the molecular processes taking place during effector‐ and toxin‐triggered cell death in maize. We provide a framework of components involved in the initiation and execution of cell death in maize, which will enable the identification of common elements with conserved functions in pathogen‐induced cell death across plant species. [ABSTRACT FROM AUTHOR]