A novel approach to modify Stenotrophomonas sp. D6 by regulating the salt composition in the growth medium: Enhanced removal performance of Cr(VI).

In this study, a novel and effective modified microbial reducing agent was developed to detoxify Cr(VI) from aqueous solutions. This was achieved by carefully controlling specific salt components in the growth medium. Based on the single-salt modification, several effective modified salts were selected and added to the medium for synergistic modification. The results showed that the synergistic modification with NH 4 Cl and KH 2 PO 4 had the best detoxification effect on Cr(VI), reaching 98.5% at 100 mg/L Cr(VI), which was much higher than the 43.7% of the control (original Luria-Bertani medium). This enhancement was ascribed to the ability of NH 4 Cl and KH 2 PO 4 to stimulate the growth of Stenotrophomonas sp. D6 promoted chromate reductase secretion. The protein content of the modified medium supernatant was significantly increased by 10.76% compared to that before modification. Based on the micro-characterization, the main process for the elimination of Cr(VI) is microbial reduction rather than biosorption. Most of the reduced Cr was found in the extracellular suspension, thereby suggesting that the primary reduction occurred outside the cells, whereas only a small fraction was detected intracellularly. Overall, this study provides a simple and effective method for microbial treatment of heavy metals in aqueous solutions. [Display omitted] • The composition of salt in the medium was optimized to improve the removal of Cr(VI). • Enhancing reductase secretion of strains by adding NH 4 Cl and KH 2 PO 4 into medium. • Efficient reusability of the Co-modified strain lyophilized powder. [ABSTRACT FROM AUTHOR]