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Transcriptomic Analysis Reveals Novel Regulators of the Scots Pine Stilbene Pathway.

Authors :
Paasela, Tanja
Lim, Kean-Jin
Pavicic, Mirko
Harju, Anni
Venäläinen, Martti
Paulin, Lars
Auvinen, Petri
Kärkkäinen, Katri
Teeri, Teemu H
Source :
Plant & Cell Physiology. Oct2023, Vol. 64 Issue 10, p1204-1219. 16p.
Publication Year :
2023

Abstract

Stilbenes accumulate in Scots pine heartwood where they have important roles in protecting wood from decaying fungi. They are also part of active defense responses, and their production is induced by different (a)biotic stressors. The specific transcriptional regulators as well as the enzyme responsible for activating the stilbene precursor cinnamate in the pathway are still unknown. UV-C radiation was the first discovered artificial stress activator of the pathway. Here, we describe a large-scale transcriptomic analysis of pine needles in response to UV-C and treatment with translational inhibitors, both activating the transcription of stilbene pathway genes. We used the data to identify putative candidates for the missing CoA ligase and for pathway regulators. We further showed that the pathway is transcriptionally activated by phosphatase inhibitor, ethylene and jasmonate treatments, as in grapevine, and that the stilbene synthase promoter retains its inducibility in some of the tested conditions in Arabidopsis, a species that normally does not synthesize stilbenes. Shared features between gymnosperm and angiosperm regulation and partially retained inducibility in Arabidopsis suggest that pathway regulation occurs not only via ancient stress-response pathway(s) but also via species-specific regulators. Understanding which genes control the biosynthesis of stilbenes in Scots pine aids breeding of more resistant trees. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00320781
Volume :
64
Issue :
10
Database :
Academic Search Index
Journal :
Plant & Cell Physiology
Publication Type :
Academic Journal
Accession number :
173037446
Full Text :
https://doi.org/10.1093/pcp/pcad089