Design of triple-layer films with blackseed protein as dispersion or emulsion.

• Blackseed by-product protein isolate (BBPI) was produced with a yield of 73% protein. • BBPI showed the highest stability and encapsulation at pH10 and pH12 value. • A method to structure the triple-layer films was developed. • Triple-layer films was enriched with BBPI dispersion or emulsion. • BBPI improved UV–Vis barrier and increased antioxidant activity for DPPH parameter. The aim of study was to produce the blackseed protein for applications as a protein dispersion alone or an emulsifier to design of multi-layer film based on furcellaran and chitosan. The protein extraction of blackseed by-product resulted in a blackseed by-product protein isolate (BBPI) with a yield of 73% protein. The obtained BBPI resulted in a high polyphenols content (1543 mg/ml), antioxidant property (DPPH, 67%) and emulsification properties (encapsulation up to 96%), leading to a stable emulsions at pH 10 and 12. For the first time, innovative, active triple-layer films have been developed in the 1st furcellaran layer with BBPI stabilised emulsion or BBPI aqueous extract alone, followed by a 2nd layer of furcellaran and citric acid matrix, and a 3rd layer of chitosan and gelatin matrix. Compared to the control, the tested films showed improved the water behaviour, UV–Vis barrier and increased antioxidant activity for the DPPH parameter (>70%). [ABSTRACT FROM AUTHOR]