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The Gma-miR394a/GmFBX176 module is involved in regulating the soybean (Glycine max L.) response to drought stress.
- Source :
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Plant Science . Dec2023, Vol. 337, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
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Abstract
- Drought seriously affects the yield and quality of soybean. Previous studies have shown that the gma-miR394a / GmFBX176 module regulates the response of Arabidopsis to drought stress. However, whether the gma-miR394a / GmFBX176 module is involved in the regulation of the soybean drought stress response remains unclear. Here, the function of the gma-miR394a / GmFBX176 module in the soybean drought stress response was evaluated. In soybean hairy roots, drought stress induced the transcription of gma-miR394a and inhibited the transcription of GmFBX176. GUS histochemical staining showed that transgenic GmFBX176p:GUS soybean hairy root staining was weak and that GUS transcript levels decreased under drought stress. A transient expression experiment in tobacco showed that gma-miR394a inhibited GmFBX176 transcription. Under drought stress, composite soybean plants overexpressing gma-miR394a showed increased drought resistance compared with control K599 composite soybean plants (K599); their survival rate and peroxidase activity were higher than those of K599, and their malondialdehyde content was lower. In contrast, composite soybean plants overexpressing GmFBX176m3 (gma-miR394a complement site mutation) presented lower drought resistance than K599 plants. Transcriptomic sequencing showed that the gma-miR394a / GmFBX176 module affected the transcript levels of stress response genes and transcription factors. These results indicate that the gma-miR394a / GmFBX176 module can be used to improve the drought resistance of soybean. • gma-miR394a is upregulated and its target GmFBX176 is downregulated in 10% PEG-simulated drought-stressed soybean hairy roots. • Overexpression of gma-miR394a increased the tolerance of composite soybean plants to drought stress, while overexpression of GmFBX176m3 decreased the tolerance of composite soybean plants to drought stress. • Under PEG treatment, the gma-MIR394a / GmFBX176 module regulates the transcript levels of stress-related genes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01689452
- Volume :
- 337
- Database :
- Academic Search Index
- Journal :
- Plant Science
- Publication Type :
- Academic Journal
- Accession number :
- 173278385
- Full Text :
- https://doi.org/10.1016/j.plantsci.2023.111879