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Tuning polymer–blood and polymer–cytoplasm membrane interactions by manipulating the architecture of poly(2-oxazoline) triblock copolymers.

Authors :
Lobaz, Volodymyr
Liščáková, Veronika
Sedlák, František
Musil, Dominik
Petrova, Svetlana Lukáš
Šeděnková, Ivana
Pánek, Jiří
Kučka, Jan
Konefał, Rafał
Tihlaříková, Eva
Neděla, Vilém
Pankrác, Jan
Šefc, Luděk
Hrubý, Martin
Šácha, Pavel
Štěpánek, Petr
Source :
Colloids & Surfaces B: Biointerfaces. Nov2023, Vol. 231, pN.PAG-N.PAG. 1p.
Publication Year :
2023

Abstract

Bioactive moieties designed to bind to cell membrane receptors benefit from coupling with polymeric carriers that have enhanced affinity to the cell membrane. When bound to the cell surface, such carriers create a "2D solution" of a ligand with a significantly increased concentration near a membrane-bound receptor compared to a freely water-soluble ligand. Bifunctional polymeric carriers based on amphiphilic triblock copolymers were synthesized from 2-pent-4-ynyl oxazoline, 2-nonyl oxazoline and 2-ethyl oxazoline. Their self-assembly and interactions with plasma proteins and HEK 293 cells were studied in detail. The affinity of these triblock copolymers to HEK 293 cell membranes and organ tissues was tunable by the overall hydrophobicity of the polymer molecule, which is determined by the length of the hydrophobic and hydrophilic blocks. The circulation time and biodistribution of three representative triblock copolymers were monitored after intravenous administration to C57BL/6 albino mice. A prolonged circulation time was observed for polymers with longer hydrophobic blocks, despite their molecular weight being below the renal threshold. [Display omitted] • Amphiphilic polyoxazolines are versatile platforms for carrying the bioactive moieties. • Hydrophobicity of polyoxazolines is adjusted by the length of individual blocks. • Affinity of polyoxazolines to proteins and cells is determined by their hydrophobicity. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
09277765
Volume :
231
Database :
Academic Search Index
Journal :
Colloids & Surfaces B: Biointerfaces
Publication Type :
Academic Journal
Accession number :
173318591
Full Text :
https://doi.org/10.1016/j.colsurfb.2023.113564