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Role of crustacean female sex hormone in regulating immune response in the mud crab, Scylla paramamosain.
- Source :
-
Fish & Shellfish Immunology . Nov2023, Vol. 142, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Crustacean female sex hormone (CFSH) is responsible for sexual differentiation in crustaceans. The CFSH exhibited an interleukin-17 domain homologous to vertebrate IL-17, a family of inflammatory cytokines that play vital roles in immune defense. However, the immunoregulation of CFSH in crustaceans is a mystery. Therefore, this study aimed to investigate the immune regulatory roles of CFSH and CFSHR in the mud crab Scylla paramamosain. This study's immunofluorescence result revealed that Sp -CFSHR was highly expressed in granulocytes and semi-granulocytes but had moderate expression in hyalinocytes. The expression level of Sp-CFSH transcript in eyestalk ganglia and Sp - CFSHR in hemocytes were significantly up-regulated by the Poly (I:C) stimulation but significantly down-regulated in response to the lipopolysaccharide (LPS) stimulation. In our study, in vitro experiment exhibited that the nuclear transcription factors NF-κB signaling molecules (Sp-Dorsal and Sp-Relish), Sp-STAT , apoptosis-related gene Sp-IAP , and phagocytosis related gene (Sp-Rab5) expressions were significantly increased in hemocytes by recombinant CFSH (rCFSH) in vitro , but the pro-inflammatory cytokine gene (Sp-IL-16) expression was significantly suppressed. Finally, the rCFSH injection significantly up-regulated Sp-Dorsal , Sp-Relish , Sp-IAP , Sp-Caspase , Sp-ALF2 , and C-type lectin (Sp-CTL-B) expressions in hemocytes as well as enhanced the bacterial clearance of the mud crab. In conclusion, our results suggested that CFSH may be a counterpart of vertebrate IL-17 in crustaceans that can enhance innate immunity to defense against Vibrionaceae infection via the NF-κB and/or JAK-STAT signaling pathways. This study provides the first evidence that CFSH is involved in the immunoregulation in crustaceans and enriches the insight of neuroendocrine-immune regulatory system, which providing new ideas for disease prevention in the mud crab industry. • The Sp-CFSHR mRNA is highly expressed in semigranulocytes and granulocytes. • The Sp-CFSH and Sp-CFSHR mRNA respond to the challenge of Poly (I: C) and LPS. • rCFSH enhances phagocytosis of hemocytes and activates immune effector moleculesin mud crabs. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SCYLLA (Crustacea)
*SEX hormones
*IMMUNE response
*JAK-STAT pathway
Subjects
Details
- Language :
- English
- ISSN :
- 10504648
- Volume :
- 142
- Database :
- Academic Search Index
- Journal :
- Fish & Shellfish Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 173319292
- Full Text :
- https://doi.org/10.1016/j.fsi.2023.109094