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Ultrasensitive and selective detection of sulfamethazine in milk via a Janus-labeled Au nanoparticle-based surface-enhanced Raman scattering-immunochromatographic assay.
- Source :
-
Talanta . Jan2024, Vol. 267, pN.PAG-N.PAG. 1p. - Publication Year :
- 2024
-
Abstract
- Sulfamethazine (SM2) is an antibacterial drug,which has been extensively used in human and veterinary medicine, long-term consumption of which may lead to the accumulation of sulfonamides in the body. Detection of sulfonamides often uses microbiological approaches, mass spectrometry and chromatography, which are expensive and time-consuming. Surface-enhanced Raman scattering-based immunochromatographic assay (SERS-ICA) has been recently applied in the detection. Herein, a Janus-labeled Au nanoparticle with subnanosized SiO 2 -monoclonal antibody and SERS reporter (DTNB) modified simultaneously (mAbAuNpDTNB) has been developed in a SERS-based lateral flow immunosensor, which can be used for rapid, quantitative and ultrasensitive detection of sulfamethazine residue in milk. The mAbAuNpDTNB exhibits a specific array on a paper stripe, which not only identifies sulfamethazine but also straightforwardly exposes the Raman reporter between the AuNps via self-assembly. The detection sensitivity of SERS-ICA for sulfamethazine reached 0.1 pg/mL, which was far below the previously published value by ELISA and the maximum residue limit set by the European Union. The entire SERS-ICA detection for sulfamethazine was completed within 15 min. Furthermore, high accuracy for this assay was exhibited in the spiking experiment with a recovery percentage of 88.1%–112.7%. The results demonstrated that this SERS-ICA can potentially be applied in point-of-care testing as an ultrasensitive and quantitative to semi-quantitative analytical method. [Display omitted] • A Janus-labeled Au nanoparticle (mAbAuNpDTNB) by a SERS-LFIA was reported for the detecting of Sulfamethazine in milk. • The mAbAuNpDTNB exhibits specific array on paper stripe. • The mAbAuNpDTNB recognizes Sulfamethazine and can directly expose Raman reporters between AuNps through self-assembly. • The detection of the SERS-ICA for Sulfamethazine achieved a sensitivity of 0.1 pg/mL. [ABSTRACT FROM AUTHOR]
- Subjects :
- *SULFAMETHAZINE
*GOLD nanoparticles
*MELAMINE
*SERS spectroscopy
Subjects
Details
- Language :
- English
- ISSN :
- 00399140
- Volume :
- 267
- Database :
- Academic Search Index
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 173434710
- Full Text :
- https://doi.org/10.1016/j.talanta.2023.125208