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Immobilization of laccase on PAMAM dendrimers modified core-shell Fe3O4-SiO2 magnetic composite particles for degradation of 2,4-dichlorophenol.
- Source :
-
Colloids & Surfaces A: Physicochemical & Engineering Aspects . Dec2023, Vol. 679, pN.PAG-N.PAG. 1p. - Publication Year :
- 2023
-
Abstract
- Polyaminoamide (PAMAM) dendrimers with different generations from 1 G to 5 G were grafted on magnetic core-shell Fe 3 O 4 -SiO 2 composite particles, respectively. Using Fe 3 O 4 as a magnetic center and SiO 2 as the connecting layer, the PAMAM dendrimers with amino functional groups were modified on SiO 2 successfully, while laccase was immobilized to the surface of Fe 3 O 4 -SiO 2 -PAMAM x by covalent cross-linking using amide bond. Under the optimal conditions, the loading capacity of laccase reached 65.5 mg/g, and about 62.8 % of its initial activity of free laccase was reserved. Immobilized laccase degradated 2,4-dichlorophenol (2,4-DCP) with a degradation ratio of 91.5 % after 24 h. The degradation ratio of 82.9 % was maintained after 5 cycles of the operation, and when enhanced to the 10 cycles degradation, the degradation rate was still remained close to 80 %. Compared with free laccase, the comprehensive stability of immobilized laccase was improved significantly, and it displayed the excellent catalytic degradation ability for 2,4-DCP. [Display omitted] [ABSTRACT FROM AUTHOR]
- Subjects :
- *LACCASE
*IRON oxides
*MAGNETIC particles
*DENDRIMERS
Subjects
Details
- Language :
- English
- ISSN :
- 09277757
- Volume :
- 679
- Database :
- Academic Search Index
- Journal :
- Colloids & Surfaces A: Physicochemical & Engineering Aspects
- Publication Type :
- Academic Journal
- Accession number :
- 173630174
- Full Text :
- https://doi.org/10.1016/j.colsurfa.2023.132606