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A genome assembly for the southern Pacific rattlesnake, Crotalus oreganus helleri, in the western rattlesnake species complex.
- Source :
-
Journal of Heredity . Dec2023, Vol. 114 Issue 6, p681-689. 9p. - Publication Year :
- 2023
-
Abstract
- Rattlesnakes play important roles in their ecosystems by regulating prey populations, are involved in complex coevolutionary dynamics with their prey, and exhibit a variety of unusual adaptations, including maternal care, heat-sensing pit organs, hinged fangs, and medically-significant venoms. The western rattlesnake (Crotalus oreganus) is one of the widest ranging rattlesnake species, with a distribution from British Columbia, where it is listed as threatened, to Baja California and east across the Great Basin to western Wyoming, Colorado and New Mexico. Here, we report a new reference genome assembly for one of six currently recognized subspecies, C. oreganus helleri , as part of the California Conservation Genomics Project (CCGP). Consistent with the reference genomic sequencing strategy of the CCGP, we used Pacific Biosciences HiFi long reads and Hi-C chromatin-proximity sequencing technology to produce a de novo assembled genome. The assembly comprises a total of 698 scaffolds spanning 1,564,812,557 base pairs, has a contig N50 of 64.7 Mb, a scaffold N50 of 110.8 Mb, and BUSCO complete score of 90.5%. This reference genome will be valuable for studies on the genomic basis of venom evolution and variation within Crotalus , in resolving the taxonomy of C. oreganus and its relatives, and for the conservation and management of rattlesnakes in general. [ABSTRACT FROM AUTHOR]
- Subjects :
- *CROTALUS
*RATTLESNAKES
*GENOMES
*SUBSPECIES
*SPECIES
*BASE pairs
*GENOMICS
Subjects
Details
- Language :
- English
- ISSN :
- 00221503
- Volume :
- 114
- Issue :
- 6
- Database :
- Academic Search Index
- Journal :
- Journal of Heredity
- Publication Type :
- Academic Journal
- Accession number :
- 173631843
- Full Text :
- https://doi.org/10.1093/jhered/esad045