前列腺癌骨转移关键基因的生物信息学分析.

Objective: To explore the molecular mechanism of prostate cancer bone metastasis by bioinformatics, and to provide a new target for prevention and treatment of prostate cancer bone metastasis. Methods: The dataset GSE74685 containing 20 cases of prostate cancer bone metastasis and 69 cases of prostate cancer lymph node metastasis were retrieved and screened from the Gene Expression Database （GEO）. The Limma package of R software is used to preprocess the original data and screen differentially expressed genes （DEGs）. DEGs were analyzed by GO function enrichment and KEGG pathway enrichment analysis through the gene function annotation online tool DAVID. The protein-protein interaction （PPI） network of DEGs was constructed by using the STRING online retrieval tool and Cytoscape software. And the important modules and key genes were screened by using MCODE and cytoHubba plug-ins. The expression, prognosis and diagnostic value of key genes were verified by HCMDB and GEPIA database. Results: A total of 2 022 DEGs were selected from the dataset GSE74685, including 842 up-regulated genes and 1 180 down-regulated genes. GO functional enrichment analysis showed that DEGs were mainly enriched in the extracellular matrix organization, collagen catabolic process, response to mechanical stimulus, and positive regulation of neutrophil chemotaxis. KEGG pathway enrichment suggested that focal adhesion and ECM-receptor interaction were the main signal pathway for DEGs enrichment. Eleven key genes, EZH2, PLG, SRC, CAV1, CHD4, HIST2H2BE, KDM1A, POLR2E, VWF, APOE and THBS1 were obtained through PPI network screening. The verification of gene expression and survival analysis of 11 key genes showed that APOE and EZH2 were closely related to prostate cancer bone metastasis. Conclusion: EZH2 and APOE may be involved in the occurrence and development of prostate cancer bone metastasis, which can be used as potential therapeutic targets and provide a new research direction for the prevention and treatment of prostate cancer bone metastasis. [ABSTRACT FROM AUTHOR]